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Results of bacterial community profiling performed on the mock community and a collection of 18 human-derived microbiological specimens were generally in good agreement for both platforms however, in some cases, results differed significantly.
We found that these sequencing artifacts could be minimized by using bidirectional amplicon sequencing and an optimized flow order on the Ion Torrent platform. Read truncation was dependent on both the directionality of sequencing and the target species, resulting in organism-specific biases in community profiles. We observed comparatively higher error rates with the Ion Torrent platform and report a pattern of premature sequence truncation specific to semiconductor sequencing. We benchmarked performance by using a 20-organism mock bacterial community and a collection of primary human specimens. Here we compare the performances of two common “benchtop” sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16S rRNA (V1-V2) amplicon sequencing.
Download .x264-DEMAND Season 1 One Complete torrent or any other torrent from Video HD - TV shows category.High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity.
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